let-7c-5p regulates <i>CyclinD1</i> in fluoride-mediated osteoblast proliferation and activation

Abstract Endemic fluorosis is caused by the intake of high environmental fluoride levels, which causes dental and skeletal fluorosis. Osteoblast proliferation activation closely related to tightly regulated cell cycle. Several biological processes, including bone metabolism osteoblast activation, are a type non-coding RNA called microRNAs (miRNAs). However, understanding miRNA functions in limited. Based on our previous sequencing results bioinformatics analysis, we investigated function let-7c-5p regulate CyclinD1 fluoride-induced activation. We designed population experiments as well vitro studies using EdU, flow cytometry, immunofluorescence, dual-luciferase reporters, chromatin immunoprecipitation. The population-based analysis showed decrease expression exposure increased. In addition, levels were negatively correlated with Wnt9a (another target). verified that participates human osteoblasts directly targeting CyclinD1. Furthermore, demonstrated regulates via Wnt/β-catenin signaling pathway. This study participation regulation at post-transcriptional transcriptional levels. report for first time Wnt9a/β-catenin/CyclinD1 axis involved development.